Heteroduplex Analysis
| Definition: |
A method of detecting gene mutation by mixing PCR-amplified mutant and wild-type DNA followed by denaturation and reannealing. The resultant products are resolved by gel electrophoresis, with single base substitutions detectable under optimal electrophoretic conditions and gel formulations. Large base pair mismatches may also be analyzed by using electron microscopy to visualize heteroduplex regions. |
| Previously Indexed: |
Nucleic Acid Heteroduplexes (1980-1998),Nucleic Acid Hybridization (1973-1979) |
Heteroduplex Analysis Definitions and Terms
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