Nucleic Acid Hybridization
| Definition: |
Widely used technique which exploits the ability of complementary sequences in single-stranded DNAs or RNAs to pair with each other to form a double helix. Hybridization can take place between two complimentary DNA sequences, between a single-stranded DNA and a complementary RNA, or between two RNA sequences. The technique is used to detect and isolate specific sequences, measure homology, or define other characteristics of one or both strands. (Kendrew, Encyclopedia of Molecular Biology, 1994, p503; Dorlands, 28th ed, p781) |
| Notes: |
IM general only; coord NIM with DNA or RNA or specific (IM); IN SITU HYBRIDIZATION & IN SITU HYBRIDIZATION, FLUORESCENCE are available |
| Also Called: |
Genomic Hybridization |
| Previously Indexed: |
DNA (1966-1970),RNA (1966-1970) |
Nucleic Acid Hybridization Categories.
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Heteroduplex Analysis - A method of detecting gene mutation by mixing PCR-amplified mutant and wild-type DNA followed by denaturation and reannealing. The resultant products are resolved by gel electrophoresis, with single base substitutions detectable under optimal electrophoretic conditions and gel formulations. Large base pair mismatches may also be analyzed by using electron microscopy to visualize heteroduplex regions. |
Nucleic Acid Hybridization Definitions and Terms
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