Molecular Probe Techniques
| Definition: |
The use of devices which use detector molecules to detect, investigate, or analyze other molecules, macromolecules, molecular aggregates, or organisms. |
| Notes: |
specific probes are indented under MOLECULAR PROBES in Cat D26; DF: MOL PROBE TECHNIQUES |
Molecular Probe Techniques Categories.
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Biotinylation - Incorporation of biotinyl groups into molecules. |
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Blotting, Southwestern - A method that is used to detect DNA-protein interactions. Proteins are separated by electrophoresis and blotted onto a nitrocellulose membrane similar to Western blotting (BLOTTING, WESTERN) but the proteins are identified when they bind labeled DNA PROBES (as with Southern blotting (BLOTTING, SOUTHERN)) instead of antibodies. |
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Branched DNA Signal Amplification Assay - A molecular probe technique that utilizes branched DNA (bDNA) as a means to amplify the hybridization signal. One end of the bDNA molecule is designed to bind a specific target, while the other end of the bDNA molecule contains many branches of DNA that are designed to bind a probe used for signal detection. |
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Oligonucleotide Array Sequence Analysis - Hybridization of a nucleic acid sample to a very large set of oligonucleotide probes, which are attached to a solid support, to determine sequence or to detect variations in a gene sequence or expression or for gene mapping. |
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Protein Array Analysis - Ligand-binding assays that measure protein-protein, protein-small molecule or protein-nucleic acid interactions using a very large set of capturing molecules, i.e., those attached separately on the solid support, to measure the presence or interaction of target molecules in the sample. |
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Random Amplified Polymorphic DNA Technique - Technique that utilizes low-stringency polymerase chain reaction (PCR) amplification with single primers of arbitrary sequence to generate strain-specific arrays of anonymous DNA fragments. RAPD technique may be used to determine taxonomic identity, assess kinship relationships, analyze mixed genome samples, and create specific probes. |
Molecular Probe Techniques Definitions and Terms
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