Cloning, Molecular
| Definition: |
The insertion of recombinant DNA molecules from prokaryotic and/or eukaryotic sources into a replicating vehicle, such as a plasmid or virus vector, and the introduction of the resultant hybrid molecules into recipient cells without altering the viability of those cells. |
| Notes: |
differentiate from CLONE CELLS and CLONING, ORGANISM; "clonal analysis": index under CLONE CELLS; DF: CLONING MOL or MOL CLONING |
| Previously Indexed: |
DNA (1966-1976),DNA, Recombinant (1977-1979),Plasmids (1975-1979) |
Cloning, Molecular Categories.
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Gene Fusion - Fusion of structural genes to analyze protein behavior or fusion of regulatory sequences with structural genes to determine mechanisms of regulation. |
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Two-Hybrid System Techniques - Screening techniques used to identify genes encoding interacting proteins. Variations are used to evaluate complex interplay between proteins and other molecules. |
Cloning, Molecular Definitions and Terms
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