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Tissue Culture

Definition: Maintaining or growing of tissue, organ primordia, or the whole or part of an organ in vitro so as to preserve its architecture and/or function (Dorland, 28th ed). Tissue culture includes both ORGAN CULTURE and CELL CULTURE.
Notes: NIM; Manual 18.7.2-.3, 22.26.1, 26.23+; ORGAN CULTURE & CELL CULTURE are also available

Tissue Culture Categories.
Cell Culture - A technique for maintenance or growth of animal cells in vitro. It refers to cultures derived from dispersed cells taken from the original tissue, from a primary culture, or from a cell line or cell strain by enzymatic, mechanical, or chemical disaggregation. (From Dorland, 28th ed; Freshney, Culture of Animal Cells, 3d ed, p1)
Coculture - The culturing of normal cells or tissues with infected or latently infected cells or tissues of the same kind (From Dorland, 28th ed, entry for cocultivation). It also includes culturing of normal cells or tissues with other normal cells or tissues.
Organ Culture - A technique for maintenance or growth of animal organs in vitro. It refers to three-dimensional cultures of undisaggregated tissue retaining some or all of the histological features of the tissue in vivo. (Freshney, Culture of Animal Cells, 3d ed, p1)
Tissue Engineering - Generating tissue in vitro for clinical applications, such as replacing wounded tissues or impaired organs. A cell culture facility is required for cell harvest and expansion.

Tissue Culture Definitions and Terms

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