Electrophoresis
| Definition: |
An electrochemical process in which macromolecules or colloidal particles with a net electric charge migrate in a solution under the influence of an electric current. |
| Notes: |
GEN or unspecified; prefer specifics; NIM |
| Also Called: |
Isotachophoresis |
Electrophoresis Categories.
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Electrophoresis, Agar Gel - Electrophoresis in which agar or agarose gel is used as the diffusion medium. |
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Electrophoresis, Capillary - A highly-sensitive (in the picomolar range, which is 10,000-fold more sensitive than conventional electrophoresis) and efficient technique that allows separation of proteins, nucleic acids, and carbohydrates. (Segen, Dictionary of Modern Medicine, 1992) |
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Electrophoresis, Cellulose Acetate - Electrophoresis in which cellulose acetate is the diffusion medium. |
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Electrophoresis, Gel, Pulsed-Field - Electrophoresis in which the direction of the electric field is changed periodically. This technique is similar to other electrophoretic methods normally used to separate double-stranded DNA molecules ranging in size up to tens of thousands of base-pairs. However, by alternating the electric field direction one is able to separate DNA molecules up to several million base-pairs in length. |
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Electrophoresis, Gel, Two-Dimensional - Electrophoresis in which a second perpendicular electrophoretic transport is performed on the separate components resulting from the first electrophoresis. This technique is usually performed on polyacrylamide gels. |
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Electrophoresis, Paper - Electrophoresis in which paper is used as the diffusion medium. This technique is confined almost entirely to separations of small molecules such as amino acids, peptides, and nucleotides, and relatively high voltages are nearly always used. |
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Electrophoresis, Polyacrylamide Gel - Electrophoresis in which a polyacrylamide gel is used as the diffusion medium. |
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Electrophoresis, Starch Gel - Electrophoresis in which a starch gel (a mixture of amylose and amylopectin) is used as the diffusion medium. |
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Electrophoretic Mobility Shift Assay - An electrophoretic technique employed to assay for binding of one compound to another. Typically one compound is labeled to follow its mobility during electrophoresis. The movement of the labeled compound through the electrophoretic medium will be retarded if it is bound by the other. |
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Isoelectric Focusing - Electrophoresis in which a pH gradient is established in a gel medium and proteins migrate until they reach the site (or focus) at which the pH is equal to their isoelectric point. |
Electrophoresis Definitions and Terms
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