Chromatography
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Techniques used to separate mixtures of substances based on differences in the relative affinities of the substances for mobile and stationary phases. A mobile phase (fluid or gas) passes through a column containing a stationary phase of porous solid or liquid coated on a solid support. Usage is both analytical for small amounts and preparative for bulk amounts. |
| Notes: |
GEN: prefer specifics; NIM; on Sephadex G = GEL FILTRATION see CHROMATOGRAPHY, GEL; on DEAE Sephadex = CHROMATOGRAPHY, ION EXCHANGE; DF: CHROMATOGR |
Chromatography Categories.
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Chromatography, Gas - Fractionation of a vaporized sample as a consequence of partition between a mobile gaseous phase and a stationary phase held in a column. Two types are gas-solid chromatography, where the fixed phase is a solid, and gas-liquid, in which the stationary phase is a nonvolatile liquid supported on an inert solid matrix. |
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Chromatography, Liquid - Chromatographic techniques in which the mobile phase is a liquid. |
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Chromatography, Micellar Electrokinetic Capillary - A hybrid separation technique combining both chromatographic and electrophoretic separation principles. While the method was invented to separate neutral species, it can also be applied to charged molecules such as small peptides. |
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Chromatography, Supercritical Fluid - A CHROMATOGRAPHY method using supercritical fluid, usually carbon dioxide under very high pressure (around 73 atmospheres or 1070 psi at room temperature) as the mobile phase. Other solvents are sometimes added as modifiers. This is used both for analytical (SFC) and extraction (SFE) puposes. |
Chromatography Definitions and Terms
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